Basic Information
Name of the project / program topic: AP23488282«Development of a system for intravital evaluation and prediction of meat quality of Aberdeen Angus cattle raised in Kazakhstan».
The name of the priority area of science development for which the application is submitted 6. Sustainable development of the agro-industrial complex
The name of the specialized scientific research area: 6.5 Intensive animal husbandry
Scientific director of the project/program: Ulyanova Tatiana – PhD,
The implementation period, its duration in months. 01.01.2024 – 31.12.2026 year, duration 36 month.
The total amount of funding, and by year: 120 000,0 thousand tenge,
2024 year – 34 000,0 thousand tenge;
2025 year – 43 000,0 thousand tenge;
2026 year – 43 000,0 thousand tenge.
Objects of research, development or design:_ cattle of the Aberdeen Angus breed
The purpose of the project / program: To develop approaches to genetic testing and identify genetic markers for meat productivity and beef quality for use in breeding of Aberdeen Angus cattle bred in Kazakhstan using DNA markers associated with food raw material quality.
1. Establish a sample of Aberdeen Angus cattle and select biological material.
2. Test existing and developing methods for sample preparation and nucleic acid extraction.
3. To test the AS-PCR method for genotyping Aberdeen Angus cattle for the leptin gene (LEP) in the context of assessing meat productivity and quality of food raw materials.
4. To test the PCR-RFLP method for genotyping the thyroglobulin gene (TG5) in Aberdeen Angus cattle in relation to the evaluation of meat productivity and quality of food raw materials.
5. To test the real-time PCR, PCR-RFLP methods with allele-specific primers for genotyping Aberdeen Angus cattle for the diacylglycerol O-acyltransferase 1 gene (DGAT1) in the context of assessing meat productivity and quality of food raw materials.
6. Genotyping of Aberdeen Angus cattle bred in Kazakhstan for lipid metabolism genes.
7. To develop a PCR-SBT (nucleic acid sequencing typing) method for genotyping Aberdeen Angus cattle for the LEP, TG5 and DGAT1 genes in the context of assessing meat productivity and quality of food raw materials.
8. To characterise the genetic structure of the studied animals based on the genes for leptin, thyroglobulin and diacylglycerol O-acyltransferase 1.
9. To carry out a comparative analysis of the distribution of allelic variants and different genotypes of the LEP, TG5 and DGAT1 genes in different breeds and groups.
10. To evaluate the influence of single and complex genotypes for the leptin, thyroglobulin and diacylglycerol O-acyltransferase 1 genes on meat productivity and raw material quality and to identify genetic markers for use in breeding.
– a sample of Aberdeen Angus cattle was formed and biological material was selected;
– the existing and developed methods of sample preparation and extraction of nucleic acids have been tested;
– the AS-PCR method has been tested for genotyping Aberdeen Angus cattle by leptin (LEP) gene, in the context of assessing meat productivity and the quality of food raw materials;
– the PCR- RFLP method for genotyping Aberdeen Angus cattle by the thyroglobulin (TG5) gene has been tested in the context of assessing meat productivity and the quality of food raw materials;
– methods of Real-Time PCR, PCR- RFLP with allele-specific primers for genotyping Aberdeen Angus cattle by the diacylglycerol O-acyltransferase 1 (DGAT1) gene have been tested in the context of assessing meat productivity and the quality of food raw materials;
– genotypes of cattle of the Aberdeen Angus breed bred in Kazakhstan for lipid metabolism genes have been established;
– a PCR-SBT method (nucleic acid sequencing typing) has been developed for genotyping Aberdeen Angus cattle by the LEP, TG5 and DGAT1 genes in the context of assessing meat productivity and the quality of food raw materials;
– the genetic structure of the studied animals was characterized by the genes for leptin, thyroglobulin and diacylglycerol O-acyltransferase 1;
– a comparative analysis of the distribution of allelic variants and different genotypes of the LEP, TG5 and DGAT1 genes in different breeds and groups was carried out;
– the influence of single and complex genotypes on the genes for the leptin, thyroglobulin and diacylglycerol O-acyltransferase 1 on meat productivity and quality of raw materials was evaluated, and genetic markers for use in breeding were identified.
Degree of implementation: These studies will allow cattle breeders to breed Aberdeen Angus cattle based on the identified animals with high genetic potential of productivity and quality of products obtained from them through the use of marker-associated selection, as well as the results of the project will help determine the current state of the Aberdeen Angus breed for the development of future programs to increase its number.