BR21882180 N. Terletskaya "Development of a program for the conservation and development of the resource base of promising plants of Kazakhstan for medicine and veterinary medicine under conditions of changing climate"

Project / Program Title:

BR21882180 "Development of a program for the conservation and development of the resource base of promising plants of Kazakhstan for medicine and veterinary medicine under conditions of changing climate"

Basic Information

Project topic name: BR21882180 “Development of a program for the conservation and development of the resource base of promising plants of Kazakhstan for medicine and veterinary medicine under conditions of changing climate”.

Subsection: Study of antimicrobial, antiparasitic activity and properties of complex herbal preparation on the basis of wormwood in veterinary medicine in conditions of Western Kazakhstan.

Name of the priority direction of science development, for which the application is submitted. Rational use of water resources, fauna and flora, ecology;

Name of the specialized scientific direction for which the application is submitted, type of research: Conservation and rational use of fauna and flora.

Estimated start and completion date of the project, its duration in months. 01.09.2023 – 31.12.2025 duration 28 months.

Relevance. Development of a program to ensure the conservation and development of the resource base of promising for medicine and veterinary medicine plants of Kazakhstan under conditions of changing climate is an innovative multidisciplinary area of research, which complements and expands knowledge in the field of climatology, ecology, botany, plant physiology and pharmacology. Obtaining domestic pharmaceutical phytopreparations in sufficient quantities depends on the availability and magnitude of the resource base of official and medicinal plants. Natural ranges of plants are the result of a complex of factors – environmental conditions and ecophysiological potential of the species. Climatic changes lead to significant changes in the content of valuable biologically active substances in plants, which depend on growing conditions, phenological phase of plant development, time of collection, harvested organs. An actual and important direction of research is both maintenance of biodiversity of endemic and rare plant species in natural habitats and development of promising methods of preservation of valuable plant material in artificial conditions. Assessment of the biological effect of phytocomplexes and significant biologically active substances of potentially medicinal plants will provide valuable practical recommendations for their use in the development of new pharmaceuticals with a wide range of effects for medicine and veterinary medicine, and a comprehensive analysis of botanical and climatological data will provide a forecast on the prospects for the conservation of bioresources for the further use of target species in the domestic pharmaceutical market with the subsequent development of a series of highly effective medicines.

Bibliography list for 2023.

In accordance with the calendar plan the article in the domestic edition recommended by the UNCCD Karmaliev R. S., Nurzhanova F. Kh. S., Nurzhanova F. H., Ertleuova B. O., Sidikhov B. M. “Evaluation of antimicrobial properties of medicinal plants for the development of a complex herbal preparation”.

Rashid Sagitovich Karmaliev, Project Manager. Higher education, Doctor of Veterinary Sciences, academic title of Associate Professor. Main place of work and position – West Kazakhstan Agrarian-Technical University named after Zhangir Khan, Associate Professor of the Institute of “Veterinary Medicine and Animal Husbandry”. ORCID https://orcid.org/ 0000-0003-2565-3107 Hirsch index by Scopus 1, by RSCI 7. Scopus Author ID AU-ID 57211623292. Research interests: parasitology, pharmacology, toxicology. He has published more than 180 scientific papers, including in Scopus base editions 3 articles recommended by the COCNVO MNVO RK 9 scientific articles, 3 recommendations, 1 scientific monograph, 3 patents, 6 teaching aids , was the head of the scientific project of grant funding of the Ministry of Education and Science of the Republic of Kazakhstan: 2018 – 2020. “Development of control measures against the main helminthic diseases of cattle in steppe, semi-desert and desert zones of West Kazakhstan region depending on meteorological conditions“.

Information about the main publications of the project’s scientific supervisor Rashid Sagitovich Karmaliev:

Karmaliev, R.S. Natural and social factors of opisthorchiasis circulation in West Kazakhstan region / Nurzhanova F. H., Karmaliev R. S., Sengaliev E. S., Sengaliev E. M. // Theory and practice of the fight against parasitic diseases: Collection of scientific articles on the materials of the international scientific conference. Issue 22. May 19-21, 2021 Moscow / – M.: VNIIP – branch of FGBNU FSC VIEV RAS; Publishing House “Nauka”, 2021. – 616 с. DOI: https://doi.org/10.31016/978-5-6046256-1-3.2021.22.

Karmaliev, R.S. Invasiveness of carp fish larvae Opisthorchis felineus in West Kazakhstan region / Nurzhanova F.H., Karmaliev R.S., Sengaliev E.M., Absatirov G.G. // Russian Journal of Parasitology, 2021. 15(2), pp. 29-35. DOI: 10.31016/1998-8435-2021-15-2-29-35.

Karmaliev, R.S., Akhmedenov K.M., Sidikhov B.M., Aituganov B.E., Usenov J.T., Ertleuova B.O., Gabdullin D.E., Aliev E.M. Seasonal dynamics of strongyles of the digestive tract of cattle in the West Kazakhstan region // Ізденіster, nәтижелер – Studies, results. – 2018 – N 2 (78) – P.56-60. https://docplayer.com/110011052-Karmaliev-r-s-ahmedenov-k-m-sidihov-b-m-aytuganov-b-e-usenov-zh-t-ertleuova-b-o-gabdullin-d-e-aliev-e-m.html

Karmaliev, R.S., Krotenkov V.P., Usenov J.T., Akhmedenov K.M., Sidikhov B.M., Aituganov B.E., Ertleuova B.O., Gabdullin D.E., Beketova S.H. Helminth infestation of the digestive tract of cattle and saigas in West Kazakhstan // Proceedings of the National Academy of Sciences of the Republic of Kazakhstan. Series of agrarian sciences. – 2018 – N 5 (47) – P.35-43. http://rmebrk.kz/journals/3937/49805.pdf

Karmaliyev, R.S., Ussenov Z.T., Sidikhov B.M., Aituganov B.E., Yertleuova B.O., Gabdullin D.E.. Helminthofauna of the digestive tract of cattle and saiga in West Kazakhstan // International Journal of Engineering and Advanced Technology (IJEAT) – 2019 – V. 9, Issue 1. – October. – P. 2600-2604. percentile in Scopus database – 5. DOI: 10.35940/ijeat.A9377.109119.

Karmaliev, R.S., Akhmedenov K.M., Sidikhov B.M., Aituganov B.E., Usenov J.T., Ertleuova B.O., Gabdullin D.E., Aliev E.M. Seasonal and age dynamics of helminth infestation of cattle in different natural zones of West Kazakhstan region // Veterinary. – 2019. – N 7. – С.38-41. https://docplayer.com/110011052-Karmaliev-r-s-ahmedenov-k-m-sidihov-b-m-aytuganov-b-e-usenov-zh-t-ertleuova-b-o-gabdullin-d-e-aliev-e-m.html

Pat. 2269939 Russian Federation, MPK G01N 33/48. Method of determination of anthelmintic resistance in parasitic nematodes and in-vitro screening of preparations against them / Nikitin V.F. (RU), Karmaliev R.S. (KZ); Applicant and patentee All-Russian Research Institute of Helminthology named after K.I. Skryabin (VIGIS) (RU). K.I. Skryabin (VIGIS) (RU). – No. 2003127041/15; Procl. 05.09.2003; published 20.02.2006, Bulletin No. 17. https://gosreestr.kazpatent.kz

Karmaliev R.S. Recommendations for the prevention of piroplasmidosis transmitted by ixodal ticks in the West Kazakhstan region. // Uralsk. RIC NAO ZKATU named after Zhangir Khan, 2022. – 45 с.

Karmaliev R. S., Nametov A. M., Murzabaev K. E., Dushaeva L. J., Orynkhanov K. A., Kadralieva B. T., Karagulov A. I., Marat M. B. Antiseptics of natural, non-chemical origin, their toxicological and allergic effects on the body of laboratory animals // Science and Education. 2023, VOL. 1, NO. 3 (72), PP. 230-246. DOI: https://doi.org/10.52578/2305-9397-2023-3-1-230-246

Members of the research team:

Sidikhov Bekzhasar Mustakhapovich, higher education – West Kazakhstan Agrarian-Technical University named after Zhangir khan. Zhangir khan, academic degree of candidate of veterinary sciences of the Russian Federation. associate professor of the Institute of “Veterinary medicine and animal husbandry”. ORCID https://orcid.org/0000-0001-6471-3737. Scopus Author ID – 57211620002. Priority directions of his scientific activity are researches on epizootology, diagnostics and measures of struggle against opisthorchiasis of carnivores in West Kazakhstan region. He has published 25 works and 7 innovative patents of RK. During the last three years (2018-2020) he was the executor of the project within the grant funding of the Ministry of Education and Science of the Republic of Kazakhstan: AP05136002 “Development of control measures against the main helminthoses of cattle in steppe, semi-desert and desert zones of West Kazakhstan region depending on meteorological conditions”. He was an executor in 4 projects under grant financing of the Ministry of Education and Science of the Republic of Kazakhstan.

Nurzhanova Farida Khamidullievna, higher education – West-Kazakhstan Agrarian-Technical University named after Zhangir Khan, Master of Veterinary Sciences, postdoctoral fellow, senior lecturer of the Institute of Veterinary Medicine and Animal Husbandry. Zhangir khan, Master of Veterinary Sciences, postdoctoral fellow, senior lecturer of the Institute of Veterinary Medicine and Animal Husbandry. ORCID https://orcid.org/0000-0001-8700-6357. Scopus Author ID – 15029299700. Has experience in working with budget projects on the topics: “Assessment of epizootic situation on opisthorchiasis in water bodies of WKO and development of measures on prevention of opisthorchiasis”, “Improvement of methods of diagnostics of socially significant invasive diseases with application of new biotechnological developments”. Author of more than 45 scientific publications, including recommendations and manuals. Has 5 author’s certificates. Experience of work in the direction of research for 20 years.

Yertleuova Balausa Otargalievna, higher education, postdoctoral fellow. Main place of work and position – West Kazakhstan Agrarian-Technical University named after Zhangir Khan, senior lecturer of the Institute of “Veterinary Medicine and Animal Husbandry”. ORCID -https://orcid.org/0000-0003-1990-570X Scopus Author ID – 57211621581. She has published more than 40 scientific works, including in Scopus base publications 2 articles, recommended by the COKSNVO MNVO RK 10 scientific articles, 1 recommendation, 1 scientific monograph, 1 patent, 6 teaching aids, was the executor of two scientific projects of grant funding of the Ministry of Education and Science of the Republic of Kazakhstan: 2018 – 2020. “Development of control measures against major helminth infections of cattle in steppe, semi-desert and desert zones of West Kazakhstan region depending on meteorological conditions” and “Improving the effectiveness of breeding methods in cattle breeding“.

Project / Program Tasks

Project Goal - Development of a program to ensure the conservation and development of the resource base of promising for medicine and veterinary medicine plants of Kazakhstan in the conditions of changing climate.

Study of antimicrobial and antiparasitic activity of complex plant preparation on the basis of wormwood and other plants in veterinary medicine and its properties in the conditions of Western Kazakhstan.

Achieved Results and Novelty

For the 1st year of the project.

The antimicrobial properties of infusions of wormwood (Artemisia absinthum, Artemisia lerchiana), St. John's wort (Hypericum perforatum), succession (Bidens tripartita), celandine (Chelidonium majus), calendula (Calendula officinalis) and chamomile (Chamomilla recutita) in vitro by the method of growth retardation of test microbial culture of Staphylococcus aureus, Streptococcus and Escherichia coli on MPB and MPA. The mentioned plants have antiseptic, anticonvulsant, antimicrobial, antiparasitic, disinfectant, styptic, sedative, wound healing analgesic, anti-inflammatory, anti-allergic, sedative and local anesthetic effect. The aim of the research is to determine the antimicrobial activity of plants for the development of herbal complex preparation. Tested herbal remedies from local raw materials Artemisia absinthum, A. lerchiana, Hypericum perforatum, Bidens tripartita, Chelidonium majus, Calendula officinalis, Chamomilla recutita were used in the form of infusions, which were made by conventional methods of pharmacology. To study the antimicrobial properties of the infusions of the above plants in vitro we used the method of microbial culture growth retardation on MPB and disco-diffusion method on MPA. As a result of studies of antimicrobial activity of plant infusions on MPB and MPA, it was determined that two species of wormwood (Artemisia absinthum, Artemisia lerchiana) showed high antimicrobial activity against test microbes Staphylococcus aureus, Streptococcus and Escherichia coli. St. John's wort (Hypericum perforatum), succession (Bidens tripartita) and celandine (Chelidonium majus) showed medium antimicrobial activity. These plants were selected for the development of a complex herbal preparation. The antimicrobial activity of calendula (Calendula officinalis) and chamomile (Chamomilla recutita) was low and we do not use them further.

Expected Results

Data will be obtained on the antimicrobial and antihelminthic activity of the complex herbal preparation in veterinary medicine.

The results of clinical, biochemical, hematological studies of the complex herbal preparation on farm animals will be obtained.

The results of analysis of some toxicological properties of the complex plant preparation on farm animals will be obtained.

List of Project Publications

Karmaliev, R.S. Natural and social factors of opisthorchiasis circulation in West Kazakhstan region / Nurzhanova F. H., Karmaliev R. S., Sengaliev E. S., Sengaliev E. M. // Theory and practice of the fight against parasitic diseases: Collection of scientific articles on the materials of the international scientific conference. Issue 22. May 19-21, 2021 Moscow / - M.: VNIIP - branch of FGBNU FSC VIEV RAS; Publishing House "Nauka", 2021. - 616 с. DOI: https://doi.org/10.31016/978-5-6046256-1-3.2021.22.

Karmaliev, R.S., Akhmedenov K.M., Sidikhov B.M., Aituganov B.E., Usenov J.T., Ertleuova B.O., Gabdullin D.E., Aliev E.M. Seasonal dynamics of strongyles of the digestive tract of cattle in the West Kazakhstan region // Ізденіster, nәтижелер - Studies, results. - 2018 - N 2 (78) - P.56-60. https://docplayer.com/110011052-Karmaliev-r-s-ahmedenov-k-m-sidihov-b-m-aytuganov-b-e-usenov-zh-t-ertleuova-b-o-gabdullin-d-e-aliev-e-m.html

Karmaliev, R.S., Krotenkov V.P., Usenov J.T., Akhmedenov K.M., Sidikhov B.M., Aituganov B.E., Ertleuova B.O., Gabdullin D.E., Beketova S.H. Helminth infestation of the digestive tract of cattle and saigas in West Kazakhstan // Proceedings of the National Academy of Sciences of the Republic of Kazakhstan. Series of agrarian sciences. - 2018 - N 5 (47) - P.35-43. http://rmebrk.kz/journals/3937/49805.pdf

Karmaliyev, R.S., Ussenov Z.T., Sidikhov B.M., Aituganov B.E., Yertleuova B.O., Gabdullin D.E.. Helminthofauna of the digestive tract of cattle and saiga in West Kazakhstan // International Journal of Engineering and Advanced Technology (IJEAT) - 2019 - V. 9, Issue 1. - October. - P. 2600-2604. percentile in Scopus database - 5. DOI: 10.35940/ijeat.A9377.109119.

Karmaliev, R.S., Akhmedenov K.M., Sidikhov B.M., Aituganov B.E., Usenov J.T., Ertleuova B.O., Gabdullin D.E., Aliev E.M. Seasonal and age dynamics of helminth infestation of cattle in different natural zones of West Kazakhstan region // Veterinary. - 2019. - N 7. - С.38-41. https://docplayer.com/110011052-Karmaliev-r-s-ahmedenov-k-m-sidihov-b-m-aytuganov-b-e-usenov-zh-t-ertleuova-b-o-gabdullin-d-e-aliev-e-m.html

Pat. 2269939 Russian Federation, MPK G01N 33/48. Method of determination of anthelmintic resistance in parasitic nematodes and in-vitro screening of preparations against them / Nikitin V.F. (RU), Karmaliev R.S. (KZ); Applicant and patentee All-Russian Research Institute of Helminthology named after K.I. Skryabin (VIGIS) (RU). K.I. Skryabin (VIGIS) (RU). - No. 2003127041/15; Procl. 05.09.2003; published 20.02.2006, Bulletin No. 17. https://gosreestr.kazpatent.kz

Karmaliev R.S. Recommendations for the prevention of piroplasmidosis transmitted by ixodal ticks in the West Kazakhstan region. // Uralsk. RIC NAO ZKATU named after Zhangir Khan, 2022. - 45 с.

Farida Nurzhanova, Gaisa Absatirov, Bekzhasar Sidikhov, Alexander Sidorchuk, Nurbek Ginayatov and Kenzhebek Murzabaev. The vulnerary potential of botanical medicines in the treatment of bacterial pathologies in fish. Veterinary World, 2021. 14(3): 551-557. DOI: 10.14202/vetworld.2021.551-557

Rashid Karmaliyev, Farida Nurzhanova, Bekzhassar Sidikhov, Kenzhebek Murzabaev, Nurzhan Sariyev, Berik Satybayev and Ilana Abirova. Epizootiology of Opisthorchiasis in Carnivores, Fish and Mollusks in the West Kazakhstan Region. American Journal of Animal and Veterinary Sciences 2023, 18 (2): 147.155. DOI: 10.3844/ajavsp.2023.147.155.

Method of treatment of bacterial infections of sturgeon fish in a closed water supply unit (patent RK for invention No. 32737 dated 19.03.2018). Ministry of Justice of the Republic of Kazakhstan. Absatirov G.G., Kakishev M.G., Ginayatov N.S., Tumenov A.N., Sariev B.T., Kadralieva B.T., Nurzhanova F.H. https://gosreestr.kazpatent.kz

Method of obtaining antibacterial preparation of plant origin, possessing antibacterial, anti-inflammatory and wound healing effect (Patent RK for invention № 34450 from 10.07.2020). National Institute of Intellectual Property of the Ministry of Justice of the Republic of Kazakhstan. Absatirov G.G., Nurzhanova F.H., Sidorchuk A.A., Ginayatov N.S., Kadralieva B.T. https://gosreestr.kazpatent.kz

Patent No. 34450 Method of obtaining antibacterial preparation of plant origin, having antibacterial, anti-inflammatory and wound healing effect 10.07.2020. Applicant and patentee NAO "West-Kazakhstan Agrarian-Technical University named after Zhangir khan" Absa. Zhangir khan" Absatirov G.G. Nurzhanova F.H., Kadralieva B.T., Ginayatov N.S. https://gosreestr.kazpatent.kz

Karmaliev, R.S. Natural and social factors of opisthorchiasis circulation in West Kazakhstan region / Nurzhanova F. H., Karmaliev R. S., Sengaliev E. S., Sengaliev E. M. // Theory and practice of the fight against parasitic diseases: Collection of scientific articles on the materials of the international scientific conference. Issue 22. May 19-21, 2021 Moscow / - M.: VNIIP - branch of FGBNU FSC VIEV RAS; Publishing House "Nauka", 2021. - 616 с. DOI: https://doi.org/10.31016/978-5-6046256-1-3.2021.22.

Karmaliev, R.S., Akhmedenov K.M., Sidikhov B.M., Aituganov B.E., Usenov J.T., Ertleuova B.O., Gabdullin D.E., Aliev E.M. Seasonal dynamics of strongyles of the digestive tract of cattle in the West Kazakhstan region // Ізденіster, nәтижелер - Studies, results. - 2018 - N 2 (78) - P.56-60. https://docplayer.com/110011052-Karmaliev-r-s-ahmedenov-k-m-sidihov-b-m-aytuganov-b-e-usenov-zh-t-ertleuova-b-o-gabdullin-d-e-aliev-e-m.html

Karmaliev, R.S., Krotenkov V.P., Usenov J.T., Akhmedenov K.M., Sidikhov B.M., Aituganov B.E., Ertleuova B.O., Gabdullin D.E., Beketova S.H. Helminth infestation of the digestive tract of cattle and saigas in West Kazakhstan // Proceedings of the National Academy of Sciences of the Republic of Kazakhstan. Series of agrarian sciences. - 2018 - N 5 (47) - P.35-43. http://rmebrk.kz/journals/3937/49805.pdf

Karmaliyev, R.S., Ussenov Z.T., Sidikhov B.M., Aituganov B.E., Yertleuova B.O., Gabdullin D.E.. Helminthofauna of the digestive tract of cattle and saiga in West Kazakhstan // International Journal of Engineering and Advanced Technology (IJEAT) - 2019 - V. 9, Issue 1. - October. - P. 2600-2604. percentile in Scopus database - 5. DOI: 10.35940/ijeat.A9377.109119.

Karmaliev, R.S., Akhmedenov K.M., Sidikhov B.M., Aituganov B.E., Usenov J.T., Ertleuova B.O., Gabdullin D.E., Aliev E.M. Seasonal and age dynamics of helminth infestation of cattle in different natural zones of West Kazakhstan region // Veterinary. - 2019. - N 7. - С.38-41. https://docplayer.com/110011052-Karmaliev-r-s-ahmedenov-k-m-sidihov-b-m-aytuganov-b-e-usenov-zh-t-ertleuova-b-o-gabdullin-d-e-aliev-e-m.html

Karmaliev R.S. Recommendations for the prevention of piroplasmidosis transmitted by ixodal ticks in the West Kazakhstan region. // Uralsk. RIC NAO ZKATU named after Zhangir Khan, 2022. - 45 с.

Sergaliev, M. Kakishev , N. Ginayatov, F. Nurzhanova, E. Andronov .Microbiome structure in a recirculating aquaculture system and its connection to infections in sturgeon fish. Veterinary World, 2021. 14(3): 661-668. DOI: 10.14202/vetworld.2021.661-668

Nurzhanova F.H., Abekeshev N.T., Zhumagalieva G.K., Montayeva N.S. Epizootologic features of dog babesiosis in Uralsk. Ғylym zhane bilim. Vol.1 No.3(72) (2023): pp.123-131, DOI 10.52578/2305-9397-2023-3-1-1-122-131

Pat. 24884 Republic of Kazakhstan, MPK C 12 Q 1/02. Method of artificial infection of domestic cats with opisthorchiasis / Kereev Y.M., Shalmenov M.Sh., Nurzhanova F.H., Sidikhov B.M. (KZ); Applicant and patentee NAO "Zap.-Kaz.agr.-technical university named after Zhangir khan". Zhangir khan". - No. 219.1; filed. 04.10.2010; published 15.11.2011, Bulletin No. 11. https://gosreestr.kazpatent.kz

Pat. 24705 Republic of Kazakhstan, IPC C 12 N 1/00 (2010.01); C 12 Q 1/02. Method of artificial infection of domestic dogs with opisthorchiasis / Kereev Y.M., Shalmenov M.Sh., Nurzhanova F.H., Sidikhov B.M. [and others] (KZ); Applicant and patentee NAO "Zap.-Kaz.agr.-technical university named after Zhangir khan". Zhangir khan". - No. 2010/1325.1; filed. 26.10.2010; published 17.10.2011, Bulletin No. 10. https://gosreestr.kazpatent.kz

Pat. 032051 Republic of Kazakhstan, MPC C 12 N 1/00 C 12 Q 1/02 Method of artificial infection of hamster with opisthorchiasis / Kereev Y.M.; Shalmenov M.Sh.; Sidikhov B.M.; Nurzhanova F.H.; Lukmanova J.G.; Sariev B.T. (KZ); Applicant and patentee NAO "Zap.-Kaz.agr.-technical university named after Zhangir khan". Zhangir khan". - No. 2011/0860.1; filed. 01.08.2011; published 25.12.2012, Bulletin No. 12. https://gosreestr.kazpatent.kz

Method of treatment of bacterial infections of sturgeon fish in a closed water supply unit (RK patent for invention No. 32737 dated 19.03.2018). Ministry of Justice of the Republic of Kazakhstan. Absatirov G.G., Kakishev M.G., Ginayatov N.S., Tumenov A.N., Sariev B.T., Kadralieva B.T., Nurzhanova F.H. https://gosreestr.kazpatent.kz

Method of obtaining an antibacterial preparation of plant origin, which has antibacterial, anti-inflammatory and wound healing effect (Patent RK for invention No. 34450 dated 10.07.2020). National Institute of Intellectual Property of the Ministry of Justice of the Republic of Kazakhstan. Absatirov G.G., Nurzhanova F.H., Sidorchuk A.A., Ginayatov N.S., Kadralieva B.T. https://gosreestr.kazpatent.kz

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